Assessing population genomic structure and polyploidy: a crucial step for native plant restoration

Establishing an effective restoration program requires baseline genetic information to make sound decisions for seed increase and transfer. For many plants this is lacking, especially among native forbs that are critical pollinator health. Erigeron speciosus a widespread, perennial forb occupying montane environments in the western United States Canada. This species important fostering diversity. Our study examines population patterns across range using reduced-representation sequencing surveys genome duplication flow cytometry cytology. These genomic tools provide transfer, necessary programs. Population differentiation (FST) average was 0.13 ranged from 0.05 0.24 23 collection sites. Model-based Bayesian clustering supported model with sites grouped into two populations, distinct geographic regions of range. A distance-based neighbor-joining tree also division. Flow 53 samples 17 populations had 2C values 1.7 3.6 pg mean value 2.3 pg. Putative triploids were found individuals one site. The spatial distribution structure supports regionally based taxonomic descriptions varieties: North macranthus South. assessment describes approach developing species, those may harbor complex taxonomy polyploidy.